|
KMID : 0370019960100000195
|
|
Chung-Ang Journal of Pharmacal Sciences
1996 Volume.10 No. 0 p.195 ~ p.202
|
|
|
Detection of an endogenous inhibitor of secretory phospholipase A₂in porcine spleen
|
|
신혜숙/Shin,Hye-Sook
김대경/Kim, Dae-Kyong
|
|
|
Abstract
|
|
|
It has been known that secretory group Ⅱ phospholipase A_2 (PLA_2) plays a role in a variety of cell responses including inflammation and platelet aggregation through the production of bioactive lipid mediators such as eicosanoids and platelet activating factor. Although it has been suggested that there may be a regulatory endogenous factor which inhibits the secretory PLA_2 activity, its entity and biochemical properties has not been elucidated yet.
In the present study we detected an inhibitory protein for the secretory group Ⅱ PLA_2 in 100,000 x g supernatants obtained from the homogenates of rat platelets and porcine spleen. The inhibitory protein was bound to an anionic exchange DEAE-5PW HPLC column and did not inhibit poricine pancreatic group Ⅰ and porcine spleen 100 kDa cytosolic PLA_2 activities. suggesting that it may selectively inhibit the secretory group Ⅱ PLA_2 activity.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|